Acridine orange staining for diagnosis of Mycoplasma bovis infection in cow milk

Acridine orange staining for diagnosis of Mycoplasma bovis infection in cow milk.

Mycoplasma organisms were readily recognized in samples of milk or udder secretions from cows with clinical Mycoplasma bovis mastitis when these samples were stained with 0.01% acridine orange at pH 3.0. Samples could be stored at -4 degrees C for several days or subjected to repeated freezing and thawing without loss of staining or fluorescence properties. Use of this procedure in diagnostic l...

Histochemical observations on Mycoplasma after staining with acridine orange.

Mycoplasma colonies and Mycoplasma cells in preparations from infected milk and lymph nodes were observed for their fluorescent qualities after treatment with acridine orange. Mycoplasma colonies fluoresced brilliant red or red-orange. When treated after exposure to ribonuclease, the colonies fluoresced lime-green. There was no fluorescence when both ribonucleic acid and deoxyribonucleic acid w...

Acridine orange staining for rapid diagnosis of Acanthamoeba keratitis.

Acanthamoeba keratitis is uncommon, but one of the most severe infectious diseases of the cornea. Delayed diagnosis or misdiagnosis as bacterial or herpes simplex keratitis leads to extensive corneal inflammation and profound visual loss. Therefore, accurate and rapid diagnosis of Acanthamoeba keratitis is essential for successful treatment and good prognosis. We evaluated the usefulness of acr...

The effect of varicocele on sperm morphology and DNA maturity: does acridine orange staining facilitate diagnosis?

OBJECTIVE The aim of this study was to determine whether Acridine Orange (AO) can be used as a routine procedure in a physician's office to detect sperm deoxyribonucleic acid (DNA) fragmentation in patients with varicoceles. MATERIAL AND METHODS Forty-five patients with a mean age of 30.4 years, who had been diagnosed with varicoceles by physical examination and 30 healthy males with a mean a...

Acridine orange staining of virus infected host cells to monitor proliferation of viral infection